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KMID : 0387720120230030204
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Korean Journal of Blood Transfusion
2012 Volume.23 No. 3 p.204 ~ p.209
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Evaluation of Automated Blood Bank Systems AutoVue Innova and QWALYS-3 for ABO-RhD Grouping and Antibody Screening
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An Tae-Kyu
Song Yoon-Kyung
Seo Hee-Seoung
Kim Kang-Lim
Ko Chang-Ha
Lee Do-Hoon
Kong Sun-Young
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Abstract
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Background: Introduction of automation instruments for the blood bank is essential in order to reduce inspection
error and minimize workload. We compared the results of ABO-RhD blood type and antibody screening tests using
the manual method and those using the automation instruments AutoVue Innova (Ortho-Clinical Diagnostics,
Raritan, NJ, USA) and QWALYS-3 (DIAGAST, Loos Cedex, France).
Methods: ABO-RhD blood type tests using the slide method, the tube method, and the instruments were performed
with 200 selected samples. Antibody screening tests using the Ortho BioVue system (Ortho-Clinical Diagnostics,
Raritan, NJ, USA), which is used in our laboratory, and the two instruments were performed with 188 specimens
and 12 antibody positive samples that were kept in the laboratory. We evaluated the concordance rate of the results,
applying CLSI guideline EP12-A2.
Results: The concordance rate of ABO-RhD blood type results between the manual methods and the two
instruments was 100%. On antibody screening tests, a concordance rate of 100% was observed between the manual
method and AutoVue Innova, which uses the gel card manufactured by the company making the gel card used
for the manual method. However, using QWALYS-3 in performance of antibody screening tests, the concordance
rate was 97.5%, because of discordance in five specimens.
Conclusion: The concordance rate of ABO-RhD blood type by use of two automation instruments was 100%,
however, that of the antibody screening test was 97.5%. Thus, there was a difference in positive rate on the antibody
screening test, depending on the instrument. Therefore, introduction of an instrument, considering the pros and
cons for each instrument, is necessary. In addition, further discussion of standardized guidelines for quality control
is needed.
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KEYWORD
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Blood bank, Automation, AutoVue Innova, QWALYS-3
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